Generator

Part:BBa_K917013:Design

Designed by: Jakub Krakowiak   Group: iGEM12_Edinburgh   (2012-09-24)


pLac promoter plus cytochrome maturation gene cluster ccmA-H


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1237
    Illegal BamHI site found at 4026
    Illegal BamHI site found at 4254
    Illegal BamHI site found at 6598
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 891
    Illegal AgeI site found at 1718
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 5305


Design Notes

no special notes


Source

pLac gene comes from E. coli BL21 genomic DNA ccm gene cluster was cloned from E. coli JM109

References